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immunofluorescence flag proteintech 20543 1 ap immunoprecipitation  (Proteintech)


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    Proteintech immunofluorescence flag proteintech 20543 1 ap immunoprecipitation
    Immunofluorescence Flag Proteintech 20543 1 Ap Immunoprecipitation, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+3+3+immunofluorescence/pmc11468980__sciadv__adq6795_sm-53-14-16?v=Proteintech
    Average 96 stars, based on 1248 article reviews
    immunofluorescence flag proteintech 20543 1 ap immunoprecipitation - by Bioz Stars, 2026-08
    96/100 stars

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    Figure 1. Enhanced Expression of PD-L1 and Akt in AdMSCs. <t>Immunofluorescence</t> was employed to assess the expression levels of PD-L1 and Akt in both AdMSC and AdMSC-PDL1-Akt cells using specific probes. (A) Cell nuclei were visualized by counterstaining with DAPI, depicted in blue. Additionally, the protein abundance of both PD-L1 and Akt was ascertained in cell lysates via Western blot analysis (B) and subsequently quantified. (C) GAPDH served as an internal loading control. Scale bar: 170 µm. All data are represented as mean ± SEM. *** p < 0.01 when compared to AdMSC.
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    Figure 1. Enhanced Expression of PD-L1 and Akt in AdMSCs. <t>Immunofluorescence</t> was employed to assess the expression levels of PD-L1 and Akt in both AdMSC and AdMSC-PDL1-Akt cells using specific probes. (A) Cell nuclei were visualized by counterstaining with DAPI, depicted in blue. Additionally, the protein abundance of both PD-L1 and Akt was ascertained in cell lysates via Western blot analysis (B) and subsequently quantified. (C) GAPDH served as an internal loading control. Scale bar: 170 µm. All data are represented as mean ± SEM. *** p < 0.01 when compared to AdMSC.
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    Image Search Results


    Figure 1. Enhanced Expression of PD-L1 and Akt in AdMSCs. Immunofluorescence was employed to assess the expression levels of PD-L1 and Akt in both AdMSC and AdMSC-PDL1-Akt cells using specific probes. (A) Cell nuclei were visualized by counterstaining with DAPI, depicted in blue. Additionally, the protein abundance of both PD-L1 and Akt was ascertained in cell lysates via Western blot analysis (B) and subsequently quantified. (C) GAPDH served as an internal loading control. Scale bar: 170 µm. All data are represented as mean ± SEM. *** p < 0.01 when compared to AdMSC.

    Journal: International journal of molecular sciences

    Article Title: PD-L1 and AKT Overexpressing Adipose-Derived Mesenchymal Stem Cells Enhance Myocardial Protection by Upregulating CD25 + T Cells in Acute Myocardial Infarction Rat Model.

    doi: 10.3390/ijms25010134

    Figure Lengend Snippet: Figure 1. Enhanced Expression of PD-L1 and Akt in AdMSCs. Immunofluorescence was employed to assess the expression levels of PD-L1 and Akt in both AdMSC and AdMSC-PDL1-Akt cells using specific probes. (A) Cell nuclei were visualized by counterstaining with DAPI, depicted in blue. Additionally, the protein abundance of both PD-L1 and Akt was ascertained in cell lysates via Western blot analysis (B) and subsequently quantified. (C) GAPDH served as an internal loading control. Scale bar: 170 µm. All data are represented as mean ± SEM. *** p < 0.01 when compared to AdMSC.

    Article Snippet: Serial cross-sections of myocardial tissues were subjected to immunofluorescence staining employing antibodies against Caspase-3 (PROTEINTECH, Chicago, IL, USA), CD25 (Abcam, Cambridge, UK), NF-κB (Abcam, Cambridge, UK), and myosin heavy chain (Leica, Wetzlar, Germany).

    Techniques: Expressing, Immunofluorescence, Quantitative Proteomics, Western Blot, Control